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- How did its nutritional requirements facilitate this research? Wild-type Neurospora has modest food requirements. It can grow in the laboratory on a simple solution of inorganic salts, glucose, and the vitamin biotin, incorporated into agar, a support medium. From this minimal medium, the mold cells use their metabolic pathways to produce all the other molecules they need. Mutating the wild type allowed Beadle and Tatum to identify metabolic defects. How were Neurospora spores treated to increase the mutation rate? The spores were treated with radioactivity. Outline the technique used to identify and isolate mutant fungi.!Link: http://users.stat.ufl.edu/~rrandles/sta4930/4930lectures/chapter6/chapter6R.pdf
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- Because each of their mutants was mutated in a single gene, they concluded that each mutated gene must normally dictate the production of one enzyme. Cite two significant findings that resulted from the research of Beadle and Tatum. Because each mutant was defective in a single gene, Beadle and Tatum saw that, taken together, the collected results provided strong support for a working hypothesis they had proposed earlier. The one gene, one enzyme hypothesis states that the function of a gene is to dictate the production of a specific enzyme. What revision of detail but not of basic principle did this hypothesis undergo as more information was gained?Link: https://answers.yahoo.com/question/index?qid=20090103193300AACBGjB
- Not all proteins are enzymes! Many proteins are constructed from two or more different polypeptide chains, and each polypeptide is specified by its own gene. Beadle and Tatum s hypothesis was therefore restated as the one gene, one polypeptide hypothesis. RNA is chemically similar to DNA, except that it contains ribose instead of deoxyribose as its sugar and has the nitrogenous base uracil rather than thymine. An RNA molecule usually consists of a single strand. Of proteins? The monomers of proteins are amino acids. Discuss transcription and translation, which are essential to the function of a protein.Link: https://uk.answers.yahoo.com/question/index?qid=20080601161954AAa1rBY
- The two nucleic acids are written in different forms of the same language, and the information is simply transcribed, or rewritten, from DNA to RNA. Just as a DNA strand provides a template for making a new complementary strand during DNA replication, it can also serve as a template for assembling a complementary sequence of RNA nucleotides. For a protein-coding gene, the resulting RNA molecule is a faithful transcript of the gene s protein-building instructions. Translation is the synthesis of a polypeptide using the information in the mrna. During this stage, there is a change in language: the cell must translate the nucleotide sequence of an mrna molecule into the amino acid sequence of a polypeptide. The sites of translation are ribosomes, complex particles that facilitate the orderly linking of amino acids into polypeptide chains. Complete the table to summarize each process. What is the pre-mrna called in eukaryotes? The initial RNA transcript from any gene, including those specifying RNA that is not translated into protein, is more generally called a primary transcript.Link: https://liveyojana.com/tag/awm/
- What is the central dogma of molecular genetics, as proclaimed by Francis Crick? Genes program protein synthesis via genetic messages in the form of messenger RNA. Cells are governed by a molecular chain of command with a directional flow of genetic information: DNA g RNA g protein How many nucleotide bases are there? There are four nucleotide bases and 20 amino acids. Three nucleotides are required to code for these 20 amino acids. So, the language of DNA is a triplet code, and 64 unique triplets exist. DNA is double-stranded, but for each protein, only one of these two strands is used to produce an mrna transcript. The template strand provides the pattern, or template, for the sequence of nucleotides in an RNA transcript. For any given gene, the same strand is used as the template every time the gene is transcribed. For other genes on the same DNA molecule, however, the opposite strand may be the one that always functions as a template.Link: https://mriquestions.com/index.html
- Label one codon. Describe Nirenberg s experiment in which he identified the first codon. Nirenberg synthesized an artificial mrna by linking identical RNA nucleotides containing uracil as their base. No matter where this message started or stopped, it could contain only one codon in repetition: UUU. Nirenberg added this poly-u to a test-tube mixture containing amino acids, ribosomes, and the other components required for protein synthesis.Link: https://courts.state.md.us/sites/default/files/import/ble/examanswers/2013/201302outofstateattorneyanalysis.pdf
- On a strand of mrna, the reading frame is the left to right 5 to 3 triplet grouping of nucleotides used by the cell s protein-synthesizing machinery. Name the enzyme that uses the DNA template strand to transcribe a new mrna strand. Both enzymes can assemble a new polynucleotide only in the 5 left to right direction. However, RNA polymerase does not require a primer to begin synthesis. What is a transcription unit? In bacteria, the RNA polymerase itself specifically recognizes and binds to the promoter.Link: https://indico.cern.ch/event/569714/contributions/2303878/attachments/1611066/2558195/Answers_Vacuum_Systems_2017.pdf
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Saturday, April 24, 2021
Chapter 17 From Gene To Protein Reading Guide Answers
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